{"id":10266,"date":"2010-02-01T09:59:58","date_gmt":"2010-02-01T07:59:58","guid":{"rendered":"http:\/\/109.232.216.219\/~bilimvegelecek\/?p=10266"},"modified":"2017-05-25T10:01:59","modified_gmt":"2017-05-25T07:01:59","slug":"ebola-virusunun-olumcul-sirri-bulundu","status":"publish","type":"post","link":"https:\/\/bilimvegelecek.com.tr\/index.php\/2010\/02\/01\/ebola-virusunun-olumcul-sirri-bulundu","title":{"rendered":"Ebola vir\u00fcs\u00fc\u2019n\u00fcn \u00f6l\u00fcmc\u00fcl s\u0131rr\u0131 bulundu"},"content":{"rendered":"
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Ebola Vir\u00fcs\u00fc (Elentron Mikroskopu Mikrograf\u0131, Cynthia Goldsmith, Amerikan Hastal\u0131k Kontrol\u00fc ve \u00d6nleme Merkezi)<\/figcaption><\/figure>\n

ABD\u2019nin Iowa eyaleti Ames \u015fehrindeki Iowa \u00dcniversitesi Biyokimya, Biyofizik ve Molek\u00fcler Biyoloji b\u00f6l\u00fcm\u00fcnden Yrd. Do\u00e7. Dr. Gaya Amarasinghe ba\u015fkanl\u0131\u011f\u0131ndaki ara\u015ft\u0131rma grubu taraf\u0131ndan ba\u011f\u0131\u015f\u0131kl\u0131k sisteminin zay\u0131flat\u0131lmas\u0131nda \u00f6nemli rol oynayan VP35 adl\u0131 Ebola vir\u00fcs proteinin yap\u0131s\u0131 daha \u00f6nce \u00e7\u00f6z\u00fclm\u00fc\u015ft\u00fc. Son olarak ise, yaz\u0131m\u0131za da konu olan \u00e7al\u0131\u015fmada Amarasinghe ve grubu Ebola VP35 proteininin bunu nas\u0131l yapt\u0131\u011f\u0131n\u0131 ke\u015ffetti. Vir\u00fcsler genelde h\u00fccreleri enfekte etti\u011finde, \u00e7o\u011falabilmek i\u00e7in \u00f6ncelikli olarak RNA sentezine ba\u015flarlar. E\u011fer enfekte edilen h\u00fccre sa\u011fl\u0131kl\u0131 bir h\u00fccre ise vir\u00fcs\u00fcn RNA sentezini alg\u0131lar, vir\u00fcs\u00fcn replikasyonunu engellemek ve enfeksiyonu temizlemek i\u00e7in anti-viral savunma mekanizmalar\u0131n\u0131 devreye sokar. Amaransinghe ve grubunun en son ke\u015ffetti\u011fi ise Ebola vir\u00fcs taraf\u0131ndan sentezlenen VP35 proteininin, replike olmakta olan vir\u00fcs RNA\u2019s\u0131n\u0131 maskeledi\u011fi, dolay\u0131s\u0131yla da enfekte olan h\u00fccrenin vir\u00fcs taraf\u0131ndan istila edildi\u011finin fark\u0131na varmamas\u0131 oldu.<\/p>\n

Amarasinghe konuyu k\u0131saca \u015f\u00f6yle \u00f6zetlemektedir:<\/p>\n

\u201c\u00d6zellikle Zaire\u2019den izole edilen Ebola vir\u00fcs \u0131rk\u0131n\u0131n en tehlikeli vir\u00fcs olarak nam salmas\u0131n\u0131n nedeni enfekte olan h\u00fccrelerin herhangi bir ba\u011f\u0131\u015f\u0131kl\u0131k cevab\u0131 verememesidir. Zaten Ebola ile ilgili olarak en \u00e7ok merak edilen sorulardan biri enfekte olan h\u00fccrelerin neden di\u011fer vir\u00fcslere kar\u015f\u0131 geli\u015ftirdi\u011fi ba\u011f\u0131\u015f\u0131kl\u0131k cevab\u0131n\u0131n Ebola vir\u00fcs\u00fcne kar\u015f\u0131 geli\u015ftirilemedi\u011fiydi. En basit cevap ise enfekte edildi\u011fini alg\u0131lamayan bir h\u00fccrenin, buna kar\u015f\u0131 ba\u011f\u0131\u015f\u0131kl\u0131k cevab\u0131 da geli\u015ftiremeyece\u011fidir. Dolay\u0131s\u0131yla bizim \u00e7al\u0131\u015fmam\u0131z Ebola enfeksiyonu ve h\u00fccre istilas\u0131 mekanizmalar\u0131n\u0131n ayd\u0131nlat\u0131lmas\u0131na b\u00fcy\u00fck katk\u0131 sa\u011flamaktad\u0131r.\u201d<\/p>\n

Amarasinghe\u2019in ara\u015ft\u0131rmay\u0131 tak\u0131m olu\u015fturarak yapma iste\u011fi New York \u015eehrindeki Mt. Sinai T\u0131p Fak\u00fcltesinden virolog Christopher Basler ile bir araya gelmesini, dolay\u0131s\u0131yla yap\u0131sal bulgular\u0131n viral proteinlerin h\u00fccre i\u00e7erisindeki fonksiyonlar\u0131 ile nas\u0131l \u00f6rt\u00fc\u015ft\u00fc\u011f\u00fcn\u00fc ortaya \u00e7\u0131karmas\u0131n\u0131 sa\u011flam\u0131\u015f. Amarasinghe daha sonra \u201cVP35 ile ilgili 2008\u2019de yapt\u0131\u011f\u0131m\u0131z, ilk yap\u0131sal \u00e7\u00f6z\u00fcmlemeler ara\u015ft\u0131rman\u0131n ilerlemesinde anahtar rol oynasa da bu denklemin sadece bir par\u00e7as\u0131n\u0131 olu\u015fturuyordu. Bu yap\u0131sal bulgular\u0131, fonksiyonel bulgular\u0131m\u0131zla bir araya getirdi\u011fimizde ise, denklemin \u00e7\u00f6z\u00fcm\u00fc noktas\u0131nda ta\u015flar yerine oturmaya ba\u015flad\u0131.\u201d diyerek \u00e7al\u0131\u015fmas\u0131 hakk\u0131ndaki bilgileri payla\u015fmaya devam etmi\u015ftir.<\/p>\n

Amarasinghe\u2019in fonksiyonel protein \u00e7al\u0131\u015fmalar\u0131yla bula\u015f\u0131c\u0131 olmayan VP35 proteinini kullanarak \u00e7\u00f6zd\u00fc\u011f\u00fc protein-RNA olu\u015fumunu tan\u0131mlayan son ara\u015ft\u0131rmas\u0131 Nature Structural and Molecular Biology<\/em> adl\u0131 bilimsel derginin 17 Ocak 2010 tarihli online versiyonunda yay\u0131nlanm\u0131\u015ft\u0131r. Bu yay\u0131ndaki \u00e7al\u0131\u015fma ise temellerini Amarasinghe\u2019in ge\u00e7en Ocak\u2019ta bilim d\u00fcnyas\u0131ndaki bir\u00e7ok ilki ve \u00f6nemli geli\u015fmeyi yay\u0131nlayan, en sayg\u0131de\u011fer bilim dergilerinden biri olan ABD\u2019nin Ulusal Bilimler Akademisindeki \u0130lerlemeler (PNAS) adl\u0131 dergiden al\u0131yor. Amarasinghe, derlememize konu olan \u00e7al\u0131\u015fmas\u0131nda ise Zaire Ebola vir\u00fcs\u00fcn\u00fcn VP35 proteininin, s\u0131ra d\u0131\u015f\u0131 g\u00f6r\u00fcnen belirli bir noktas\u0131na odaklanm\u0131\u015f. Ara\u015ft\u0131rma s\u0131ras\u0131nda test etti\u011fi hipotezler ile ilgili olarak veriler eline geldik\u00e7e, VP35 proteininin \u015f\u00fcpheli yani s\u0131ra d\u0131\u015f\u0131 k\u0131sm\u0131n\u0131n, enfekte etti\u011fi h\u00fccrede ba\u011f\u0131\u015f\u0131kl\u0131k sistemini tetikleyen molek\u00fcllere ba\u011flanmay\u0131 sa\u011flayan (ba\u011f\u0131\u015f\u0131kl\u0131k bilimi terimiyle n\u00f6tralize eden) b\u00f6lge oldu\u011funu g\u00f6rm\u00fc\u015ft\u00fcr.<\/p>\n

Amarasinghe \u201cEbola\u2019n\u0131n en ilgin\u00e7 yan\u0131 ise enfekte etti\u011fi h\u00fccrelerin kendisini savunmaya ba\u015flamas\u0131na bile izin vermemesidir. B\u00f6ylece Ebola\u2019n\u0131n viral RNA\u2019s\u0131 h\u00fccreler onu tan\u0131madan gizlenmi\u015f oluyor. Bu ba\u011f\u0131\u015f\u0131kl\u0131k sistemini istila etmek i\u00e7in bilinen en g\u00fc\u00e7l\u00fc mekanizmalardan biridir\u201d diyerek kendisi i\u00e7in konunun ne kadar da ilgin\u00e7 oldu\u011funu dile getirmektedir.<\/p>\n

 <\/p>\n

KAYNAKLAR<\/strong><\/p>\n

1) ScienceDaily, 20 Ocak 2010, http:\/\/www.sciencedaily.com\u00ad \/releases\/2010\/01\/100119154721.htm<\/p>\n

2) Leung DW, Prins KC, Borek DM, et al. Structural basis for dsRNA recognition and interferon antagonism by Ebola VP35. Nat Struct Mol Biol. On-line bas\u0131m 17 Ocak 2010; http:\/\/dx.doi.org\/10.1038\/nsmb.1765 (derleme haz\u0131rland\u0131\u011f\u0131 esnada sadecce onilne versiyonu mevcuttu)<\/p>\n","protected":false},"excerpt":{"rendered":"

ABD\u2019nin Iowa eyaleti Ames \u015fehrindeki Iowa \u00dcniversitesi Biyokimya, Biyofizik ve Molek\u00fcler Biyoloji b\u00f6l\u00fcm\u00fcnden Yrd. Do\u00e7. Dr. Gaya Amarasinghe ba\u015fkanl\u0131\u011f\u0131ndaki ara\u015ft\u0131rma grubu taraf\u0131ndan ba\u011f\u0131\u015f\u0131kl\u0131k sisteminin zay\u0131flat\u0131lmas\u0131nda \u00f6nemli rol oynayan VP35 adl\u0131 Ebola vir\u00fcs proteinin yap\u0131s\u0131 daha \u00f6nce \u00e7\u00f6z\u00fclm\u00fc\u015ft\u00fc. Son olarak ise, yaz\u0131m\u0131za da konu olan \u00e7al\u0131\u015fmada Amarasinghe ve grubu Ebola VP35 proteininin bunu nas\u0131l yapt\u0131\u011f\u0131n\u0131 ke\u015ffetti. […]<\/p>\n","protected":false},"author":778,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[109,19,21],"tags":[677],"class_list":["post-10266","post","type-post","status-publish","format-standard","hentry","category-72-sayi","category-bilim-gundemi","category-biyoloji","tag-ebola"],"acf":[],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t