{"id":10722,"date":"2011-05-01T22:04:47","date_gmt":"2011-05-01T19:04:47","guid":{"rendered":"http:\/\/109.232.216.219\/~bilimvegelecek\/?p=10722"},"modified":"2017-05-29T22:09:22","modified_gmt":"2017-05-29T19:09:22","slug":"hucrelerin-goz-kamastiran-3-d-filmlerini-yapan-yeni-mikroskop","status":"publish","type":"post","link":"https:\/\/bilimvegelecek.com.tr\/index.php\/2011\/05\/01\/hucrelerin-goz-kamastiran-3-d-filmlerini-yapan-yeni-mikroskop","title":{"rendered":"H\u00fccrelerin g\u00f6z kama\u015ft\u0131ran 3-D filmlerini yapan yeni mikroskop"},"content":{"rendered":"
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Resim Bessel I\u015f\u0131n\u0131 d\u00fczlem ayd\u0131nlatma mikroskopu kullan\u0131larak yalan bir videodan al\u0131nm\u0131\u015ft\u0131r. Bu video filopodia denilen uzun, ince ve s\u00fcrekli olarak geni\u015fleyip b\u00fcz\u00fclen \u00e7\u0131k\u0131nt\u0131lara sahip HeLa h\u00fccresinin s\u00fcrekli de\u011fi\u015fen y\u00fczeyini g\u00f6stermektedir. (Kredi: Eric Betzig\/Janelia Farm)<\/figcaption><\/figure>\n

Howard Hughes Medikal Enstit\u00fcs\u00fc Janelia Farm Ara\u015ft\u0131rma Kamp\u00fcs\u00fc bilim insanlar\u0131 taraf\u0131ndan icat edilen yeni mikroskop, ara\u015ft\u0131rmac\u0131lar\u0131n tek\/yaln\u0131z canl\u0131 h\u00fccrelerin i\u00e7ini dikkatle taray\u0131p, h\u00fccre s\u0131n\u0131rlar\u0131n\u0131n a\u00e7\u0131klay\u0131c\u0131 \u00fc\u00e7 boyutlu modelini e\u015fi benzeri g\u00f6r\u00fclmemi\u015f detaylar\u0131yla ortaya \u00e7\u0131karan -s\u00fcpermarketlerdeki ince barkod taray\u0131c\u0131lar benzeri- a\u015f\u0131r\u0131 ince \u0131\u015f\u0131k katman\u0131 kullanmalar\u0131na olanak sa\u011flayacak. Mikroskopi tekni\u011fi y\u00fcksek h\u0131zda g\u00f6r\u00fcnt\u00fcleme yapabiliyor, b\u00f6ylece ara\u015ft\u0131rmac\u0131lar h\u00fccre b\u00f6l\u00fcnmesi gibi biyolojik s\u00fcre\u00e7leri canland\u0131ran g\u00f6z kama\u015ft\u0131r\u0131c\u0131 filmler yaratabilecekler.<\/p>\n

Bessel \u0131\u015f\u0131n\u0131 d\u00fczlem ayd\u0131nlatma mikroskopisi denilen bu teknik 4 Mart 2011\u2019de Nature Methods<\/em> dergisinde online olarak yay\u0131mlanan bir ara\u015ft\u0131rma makalesinde anlat\u0131l\u0131yor.<\/p>\n

Biyologlar\u0131n ba\u015fl\u0131ca amac\u0131 h\u00fccre i\u00e7indeki molek\u00fcler s\u00fcre\u00e7lerin prensiplerini anlamak. Janelia Farm grup lideri Eric Betzig \u201cE\u011fer biri oyunun kurallar\u0131n\u0131 \u00f6\u011frenmeye \u00e7abal\u0131yorsa, bu oyunun foto\u011fraflar\u0131na bakmak yerine o oyunu oynayan insanlar\u0131n bir filmini izlemesi daha avantajl\u0131d\u0131r. Ayn\u0131 \u015fey h\u00fccreler i\u00e7in de ge\u00e7erlidir.\u201d \u015feklinde durumu a\u00e7\u0131kl\u0131yor. Kendisi 30 y\u0131ldan fazla zamand\u0131r mikroskoplar\u0131 icat etme ve geli\u015ftirme \u00fczerinde \u00e7al\u0131\u015f\u0131yor. Bu s\u00fcre i\u00e7erisinde mikroskopide b\u00fcy\u00fck geli\u015fmeler g\u00f6r\u00fclmesine ra\u011fmen, Betzig\u2019e g\u00f6re, bir\u00e7ok mikroskopi tekni\u011finin h\u00fccreleri \u00f6ld\u00fcrmeyi ve h\u00fccreyi g\u00f6r\u00fcnt\u00fcleme pozisyonuna yerle\u015ftirmeyi gerektirmesi bu alandaki bilimsel \u00e7al\u0131\u015fmalara engel oluyordu. Betzig ayn\u0131 zamanda \u201cDura\u011fan resimler gibi olan \u00f6l\u00fc h\u00fccrelerden \u00f6\u011frenilebilecek \u015feyler s\u0131n\u0131rl\u0131d\u0131r\u201d diyor.<\/p>\n

Betzig, ara\u015ft\u0131rmac\u0131lara canl\u0131 h\u00fccrelerin i\u00e7 ya\u015fam\u0131n\u0131 g\u00f6rme olana\u011f\u0131 sa\u011flayan bir mikroskop yaratmak istemi\u015f. Floresan molek\u00fcllerle i\u015faretlenmi\u015f protein ve di\u011fer molek\u00fcllerin olu\u015fturdu\u011fu noktalarca resmedilmi\u015f canl\u0131 h\u00fccrelerle \u00e7al\u0131\u015fma fikri yeni de\u011fil. Fakat canl\u0131-h\u00fccre teknikleri, mikroskop taraf\u0131ndan \u00fcretilen \u0131\u015f\u0131k zamanla h\u00fccreye zarar verebilece\u011fi i\u00e7in problemli olabilir. H\u00fccreye verdi\u011fi hasar\u0131n yan\u0131 s\u0131ra \u0131\u015f\u0131k, say\u0131s\u0131 \u00e7ok fazla olan floresan molek\u00fcllerinin zamanla parlakl\u0131\u011f\u0131n\u0131 kaybetmesine neden olur. Di\u011fer bir deyi\u015fle, h\u00fccreyle onun \u00f6zelliklerini ortaya \u00e7\u0131karmak i\u00e7in ne kadar uzun \u00e7al\u0131\u015f\u0131rsan\u0131z, h\u00fccreye o kadar \u00e7ok hasar verirsiniz ve muhtemelen bir o kadar da \u201cfoton b\u00fct\u00e7enizden\u201d harcars\u0131n\u0131z, diyor Betzig.<\/p>\n

Dahas\u0131, bir mikroskobun \u0131\u015f\u0131\u011f\u0131 numunenin odaklan\u0131lan k\u00fc\u00e7\u00fck par\u00e7as\u0131ndan daha fazlas\u0131n\u0131 \u0131\u015f\u0131kland\u0131r\u0131r. Odak d\u0131\u015f\u0131 b\u00f6lgelerin ayd\u0131nlat\u0131lmas\u0131, k\u00fc\u00e7\u00fck h\u00fccre-i\u00e7i \u00f6zelliklerin keskin noktalar yerine uzat\u0131lm\u0131\u015f lekeler olarak ortaya \u00e7\u0131kt\u0131\u011f\u0131 bulan\u0131k bir g\u00f6r\u00fcnt\u00fc \u00fcretir. Betzig\u2019e g\u00f6re buradaki soru, neden olunan zarar\u0131 minimuma indirmenin bir yolunun olup olmad\u0131\u011f\u0131. Geli\u015ftirdikleri teknikle h\u00fccrelerin fizyolojik durumlar\u0131n\u0131 bozmadan uzun zamanl\u0131 ve y\u00fcksek \u00e7\u00f6z\u00fcn\u00fcrl\u00fckl\u00fc \u00e7al\u0131\u015fma f\u0131rsat\u0131 olu\u015facak.<\/p>\n

Betzig, 2006\u2019da Janelia Farm\u2019a gitmesinden \u00e7ok \u00f6nce canl\u0131-h\u00fccre misroskopisini geli\u015ftirme yollar\u0131 d\u00fc\u015f\u00fcnmeye ba\u015flam\u0131\u015f. O, (k\u0131r\u0131n\u0131m yasalar\u0131 taraf\u0131ndan dayat\u0131lan) uzaysal \u00e7\u00f6z\u00fcnmenin limitlerine zarar verecek olan yeni mikroskopi tekniklerini tasarlamaya odaklanm\u0131\u015fken bu d\u00fc\u015f\u00fcncelerini bir s\u00fcre saklam\u0131\u015f. Yak\u0131n zamana kadar mikroskoplar 200 nanometreden daha k\u00fc\u00e7\u00fck boyutlardaki objeleri g\u00f6r\u00fcnt\u00fcleyemiyordu. Birka\u00e7 y\u0131l \u00f6nce, Betzig ve Janelia Farm\u2019dan meslekta\u015f\u0131 Herald Hess sadece 10-20 nanometre boyutlar\u0131ndaki objeleri g\u00f6r\u00fcnt\u00fcleyebilen PALM\u2019\u0131 (photoactivated localization microscopy-aktive edilmi\u015f \u0131\u015f\u0131k yerelle\u015ftirme mikroskopisi) icat etmi\u015fler.<\/p>\n

PALM ve \u00e7o\u011fu mikroskop -hatta \u00fcniversite \u00f6\u011frencilerinin biyoloji s\u0131n\u0131flar\u0131nda kulland\u0131klar\u0131 bile- numuneyi bir objektif lens \u00fczerinden \u0131\u015f\u0131\u011fa maruz b\u0131rak\u0131p daha sonra geri d\u00f6nen \u0131\u015f\u0131\u011f\u0131 ayn\u0131 lens \u00fczerinde toplamas\u0131 \u015feklinde \u00e7al\u0131\u015f\u0131r. Bu yol \u0131\u015f\u0131\u011f\u0131n numuneye zarar vermesine ve bulan\u0131kl\u0131\u011fa neden olur, bu y\u00fczden canl\u0131 h\u00fccreleri g\u00f6zlemlemeyi zorla\u015ft\u0131r\u0131r.<\/p>\n

2008 y\u0131l\u0131nda Betzig bu zorlu\u011fun \u00fcstesinden gelmenin yollar\u0131 \u00fczerinde \u00e7al\u0131\u015fmaya ba\u015flam\u0131\u015f. Sahip oldu\u011fu fikirlerden biri de d\u00fczlem ayd\u0131nlatma mikroskopisini kullanmak. \u0130lk olarak 100 y\u0131l kadar \u00f6nce \u00f6nerilen d\u00fczlem ayd\u0131nlatmas\u0131, numunenin \u00fcst k\u0131sm\u0131 yerine yan y\u00fcz\u00fcnden gelen parlayan bir \u0131\u015f\u0131k katman\u0131 i\u00e7erir. Bunu yapmak i\u00e7in mikroskop uzmanlar\u0131 birbirlerine dik iki farkl\u0131 objektif lens kullan\u0131rlar. Betzig durumu: \u201cYan k\u0131s\u0131mlardan geldi\u011finiz i\u00e7in d\u00fczlem ayd\u0131nlatma, odaklanan k\u0131sma \u00e7ok yak\u0131n olan eksitasyonu (uyar\u0131lma) s\u0131n\u0131rlar\u201d \u015feklinde a\u00e7\u0131kl\u0131yor.<\/p>\n

D\u00fczlem ayd\u0131nlatma, Janelia Farm akademi \u00fcyesi Philipp Keller de dahil olmak \u00fczere di\u011fer ara\u015ft\u0131rmac\u0131lar taraf\u0131ndan, y\u00fczlerce mikron boyutlar\u0131ndaki \u00e7ok h\u00fccreli organizmalar\u0131 incelerken etkiyi art\u0131rmada kullan\u0131lmas\u0131na ra\u011fmen, \u0131\u015f\u0131k katmanlar\u0131 sadece 10 mikron boyutundaki h\u00fccrelerin i\u00e7ini efektif \u015fekilde g\u00f6r\u00fcnt\u00fclemek i\u00e7in h\u00e2l\u00e2 \u00e7ok kal\u0131nd\u0131. As\u0131l problem, Betzig\u2019in grubunun istedi\u011finden daha fazla h\u00fccrenin d\u00fczlem ayd\u0131nlatmadaki geni\u015f \u0131\u015f\u0131k \u015feritlerine maruz kalmas\u0131yd\u0131. Bu durum a\u015f\u0131r\u0131 bulan\u0131kl\u0131\u011fa ve hafif toksisiteye sebep oluyordu. Bu problemi a\u015fmak i\u00e7in Betzig ve grubu, k\u0131r\u0131n\u0131ma u\u011framayan \u00f6zel tip \u0131\u015f\u0131k demeti olan Bessel \u0131\u015f\u0131nlar\u0131n\u0131 kullanm\u0131\u015f. Bessel \u0131\u015f\u0131nlar\u0131, 1980\u2019lerin sonunda fizik\u00e7iler taraf\u0131ndan incelenmi\u015f ve bug\u00fcn s\u00fcpermarketlerde kullan\u0131lan barkod tarama cihazlar\u0131n\u0131 da i\u00e7eren uygulamalarda kullan\u0131l\u0131yor. Betzig\u2019in grubu numune \u00fczerinde \u0131\u015f\u0131n tarama yapman\u0131n daha ince bir \u0131\u015f\u0131k katman\u0131 yaratt\u0131\u011f\u0131n\u0131 ke\u015ffetmi\u015fler.<\/p>\n

Bessel \u0131\u015f\u0131nlar\u0131 biraz garip hareket ederler, bu y\u00fczden bu \u0131\u015f\u0131nlar Betzig\u2019in doktora sonras\u0131 ara\u015ft\u0131rmac\u0131lar\u0131n\u0131 (Thomas Planchon ve Liang Gao) be\u015f y\u0131ldan fazla s\u00fcredir me\u015fgul etmekte. Bessel \u0131\u015f\u0131nlar\u0131 \u00e7ok dar \u0131\u015f\u0131k demeti \u00fcretmesine ra\u011fmen, odak noktas\u0131n\u0131n yan k\u0131sm\u0131nda bir miktar zay\u0131f \u0131\u015f\u0131k da yarat\u0131r, bu da ayd\u0131nlanma \u015fablonunun mercek \u015feklinde (bull\u2019s eye) g\u00f6r\u00fcnmesine neden olur. Ekstra \u0131\u015f\u0131k loplar\u0131 numunenin \u00e7o\u011funu harekete ge\u00e7irdi\u011fi i\u00e7in engel te\u015fkil eder. Betzig\u2019in grubu bu durumu telafi etmek i\u00e7in iki y\u00f6ntem kullanm\u0131\u015f. Bunlardan ilki, \u201cyap\u0131land\u0131r\u0131lm\u0131\u015f ayd\u0131nlatma\u201d ad\u0131ndaki konsept. Burada \u0131\u015f\u0131nlar\u0131 s\u00fcrekli olarak h\u0131zl\u0131 bir \u015fekilde yaymak yerine onlar\u0131 seri bir \u015fekilde a\u00e7\u0131p kap\u0131yorlar (makineli t\u00fcfek gibi). Bu durum odak d\u0131\u015f\u0131 bulan\u0131kl\u0131\u011f\u0131 gidermede kullan\u0131labilecek periyodik kademeli eksitasyon olu\u015fturmay\u0131 sa\u011fl\u0131yor. (Janelia Farm grup lideri Mats Gustafsson taraf\u0131ndan kullan\u0131lan yap\u0131land\u0131r\u0131lm\u0131\u015f ayd\u0131nlatma, ayr\u0131ca s\u00fcper \u00e7\u00f6z\u00fcn\u00fcrl\u00fc\u011f\u00fc elde etmenin de bir yolu.)<\/p>\n

Betzig\u2019in grubunun kulland\u0131\u011f\u0131 di\u011fer bir strateji ise iki fotonlu mikroskopi. Bu metot \u00e7o\u011funlukla n\u00f6robilimde kal\u0131n beyin dokusu par\u00e7alar\u0131n\u0131n g\u00f6rselle\u015ftirilmesinde kullan\u0131l\u0131yor. \u0130ki fotonlu mikroskoplar\u0131n avantajlar\u0131ndan biri zay\u0131f bir bi\u00e7imde \u0131\u015f\u0131\u011fa maruz b\u0131rak\u0131lan b\u00f6lgeler taraf\u0131ndan \u00e7ok k\u00fc\u00e7\u00fck floresan sinyalleri \u00fcretmesi. B\u00f6ylece iki fotonlu metot uyguland\u0131\u011f\u0131nda Bessel taraf\u0131ndaki loplar\u0131n arka plan\u0131 elenir ve geriye kalanlar da Bessel \u0131\u015f\u0131nlar\u0131n\u0131n dar merkez b\u00f6l\u00fcm\u00fcndeki \u0131\u015f\u0131kt\u0131r.<\/p>\n

Daha sonra olabildi\u011fince h\u0131zl\u0131 bir \u015fekilde g\u00f6r\u00fcnt\u00fcleme yapmaya koyulmu\u015flar. Bessel \u0131\u015f\u0131nlar\u0131 h\u0131zl\u0131 bir \u015fekilde numunenin \u00fczerinde yay\u0131l\u0131yor; b\u00f6ylece grup saniyede yakla\u015f\u0131k 200 g\u00f6r\u00fcnt\u00fc alabilmi\u015f ve y\u00fczlerce iki boyutlu g\u00f6t\u00fcnden 1-10 saniye i\u00e7inde \u00fc\u00e7 boyutlu y\u0131\u011f\u0131nlar olu\u015fturulmu\u015f. Umduklar\u0131 gibi, h\u00fccreye zarar vermeden y\u00fczlerce \u00fc\u00e7 boyutlu g\u00f6r\u00fcnt\u00fc k\u00fcmeleri elde edebilmi\u015fler. B\u00f6ylece mitoz b\u00f6l\u00fcnme gibi h\u00fccresel s\u00fcre\u00e7lerin inan\u0131lmaz filmlerini olu\u015fturabildiklerini ke\u015ffetmi\u015fler. Betzig \u201cUzaysal ve zamansal detaylar\u0131 g\u00f6r\u00fcnt\u00fclemeye bu kadar yakla\u015fabilen ba\u015fka bir teknik yok\u201d diyor.<\/p>\n

Ge\u00e7en yaz, ilk canl\u0131 h\u00fccre g\u00f6r\u00fcnt\u00fclerini elde eder etmez, Betzig, Plancho ve Gao yeni aletlerini kiral\u0131k bir spor hizmet arac\u0131na koyup onu, Ulusal Sa\u011fl\u0131k Enstit\u00fcs\u00fc\u2019nden yard\u0131mc\u0131 yazarlar Jim ve Cathy Galbraith ile birlikte \u00e7al\u0131\u015ft\u0131klar\u0131 fizyoloji dersi i\u00e7in Massachusetts\u2019teki Woods Hole Deniz Biyolojisi Laboratuvar\u0131na g\u00f6t\u00fcrm\u00fc\u015fler. Betzig, \u201cBiz burada neyin i\u015fe yaray\u0131p neyin yaramad\u0131\u011f\u0131na, g\u00f6r\u00fcnt\u00fcleme yaparken bir \u015fekilde kendi fizyolojik durumlar\u0131n\u0131 koruyan h\u00fccreleri tedavi etmenin yollar\u0131na dair bir s\u00fcr\u00fc \u015fey \u00f6\u011frendik.\u201d diyor. \u201cB\u00fct\u00fcn mikroskoplar gibi enstr\u00fcmantasyon da yapbozun sadece bir par\u00e7as\u0131d\u0131r. Bunun \u00f6nemli k\u0131sm\u0131n\u0131 ise do\u011fru numuneleri bulmak ve do\u011fru haz\u0131rlama metotlar\u0131n\u0131 kullanmak olu\u015fturur.\u201d \u015feklinde de a\u00e7\u0131klamas\u0131na devam ediyor.<\/p>\n

Yeni mikroskop heyecan verici, \u00e7\u00fcnk\u00fc gelecekte s\u00fcper \u00e7\u00f6z\u00fcn\u00fcrl\u00fckte mikroskoplar\u0131n geli\u015ftirilmesi i\u00e7in de kullan\u0131labilir. PALM ve di\u011fer s\u00fcper-\u00e7\u00f6z\u00fcn\u00fcrl\u00fck teknikleri ince ve \u00f6l\u00fc numuneleri g\u00f6zlemlemek i\u00e7in olduk\u00e7a s\u0131n\u0131rl\u0131. Ayn\u0131 zamanda canl\u0131 olanlar\u0131 g\u00f6zlemlerken de onlara zarar verebilir. Betzig bununla ilgili olarak \u201cBessel\u2019le ilgili ger\u00e7ekten m\u00fckemmel olan \u015fey ise \u015fudur; biz uyar\u0131 seviyesini (excitation) s\u0131n\u0131rlayabilir ve daha kal\u0131n yap\u0131daki h\u00fccrelerin yap\u0131lar\u0131n\u0131 ya da dinamiklerini ara\u015ft\u0131rmada s\u00fcper-\u00e7\u00f6z\u00fcn\u00fcrl\u00fck mikroskopisini uygulamay\u0131 ger\u00e7ekten d\u00fc\u015f\u00fcnmeye ba\u015flayabiliriz.\u201d diyor.<\/p>\n

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Liang Gao, Thomas Planchon ve Eric Betzig geli\u015ftirdikleri mikroskopla birlikte.<\/figcaption><\/figure>\n

S\u00fcper \u00e7\u00f6z\u00fcn\u00fcrl\u00fck olmasa bile, Bessel \u0131\u015f\u0131nlar\u0131 d\u00fczlem ayd\u0131nlatma mikroskopisi, h\u00fccre biyologlar\u0131 i\u00e7in \u00e7ok g\u00fc\u00e7l\u00fc bir ara\u00e7 olacak. Bunun nedeni, yay\u0131lma g\u00f6stermeyecek \u015fekilde, h\u00fccrelerin \u00fc\u00e7 boyutlu karma\u015f\u0131kl\u0131\u011f\u0131n\u0131 g\u00f6r\u00fcnt\u00fclemeye olanak sa\u011flamas\u0131.<\/p>\n

 <\/p>\n

Kaynak<\/strong><\/em>:<\/em> http:\/\/www.sciencedaily.com\/releases\/2011\/03\/110304151010.htm<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"

Howard Hughes Medikal Enstit\u00fcs\u00fc Janelia Farm Ara\u015ft\u0131rma Kamp\u00fcs\u00fc bilim insanlar\u0131 taraf\u0131ndan icat edilen yeni mikroskop, ara\u015ft\u0131rmac\u0131lar\u0131n tek\/yaln\u0131z canl\u0131 h\u00fccrelerin i\u00e7ini dikkatle taray\u0131p, h\u00fccre s\u0131n\u0131rlar\u0131n\u0131n a\u00e7\u0131klay\u0131c\u0131 \u00fc\u00e7 boyutlu modelini e\u015fi benzeri g\u00f6r\u00fclmemi\u015f detaylar\u0131yla ortaya \u00e7\u0131karan -s\u00fcpermarketlerdeki ince barkod taray\u0131c\u0131lar benzeri- a\u015f\u0131r\u0131 ince \u0131\u015f\u0131k katman\u0131 kullanmalar\u0131na olanak sa\u011flayacak. Mikroskopi tekni\u011fi y\u00fcksek h\u0131zda g\u00f6r\u00fcnt\u00fcleme yapabiliyor, b\u00f6ylece ara\u015ft\u0131rmac\u0131lar h\u00fccre […]<\/p>\n","protected":false},"author":804,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[124,19],"tags":[197,707],"class_list":["post-10722","post","type-post","status-publish","format-standard","hentry","category-87-sayi","category-bilim-gundemi","tag-biyoloji","tag-mikroskop"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/posts\/10722","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/users\/804"}],"replies":[{"embeddable":true,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/comments?post=10722"}],"version-history":[{"count":0,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/posts\/10722\/revisions"}],"wp:attachment":[{"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/media?parent=10722"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/categories?post=10722"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/tags?post=10722"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}