Massachusetts Teknoloji Enstit\u00fcs\u00fc (MIT) Haber B\u00fcrosu\u2019ndan Anne Trafton\u2019un kaleme ald\u0131\u011f\u0131 ara\u015ft\u0131rma haberine g\u00f6re, MIT\u2019ten bir bilim ekibi, koronavir\u00fcs ve di\u011fer enfeksiyonlar\u0131n tedavisinde kullan\u0131labilecek bir strateji olarak, sitokinlere ba\u011flanabilen antikor benzeri resept\u00f6r proteinleri tasarlad\u0131.<\/p>\n
COVID-19\u2019un belirleyici \u00f6zelliklerinden biri, ciddi vakalarda a\u015f\u0131r\u0131 ba\u011f\u0131\u015f\u0131kl\u0131k yan\u0131t\u0131 ortaya \u00e7\u0131karabilmesidir. Ayn\u0131 zamanda sitokin f\u0131rt\u0131nas\u0131 olarak da bilinen bu a\u015f\u0131r\u0131 ba\u011f\u0131\u015f\u0131kl\u0131k yan\u0131t\u0131 patlamas\u0131, akci\u011ferlere zarar verir ve \u00f6l\u00fcmc\u00fcl olabilir. MIT ekibi, a\u015f\u0131r\u0131 sitokinleri emebilece\u011fine inand\u0131klar\u0131, antikorlara benzer yap\u0131da, \u00f6zelle\u015fmi\u015f proteinler geli\u015ftirdiler.<\/p>\n
\u00c7al\u0131\u015fman\u0131n k\u0131demli yazarlar\u0131ndan, MIT ara\u015ft\u0131rmac\u0131s\u0131 Rui Qing, \u201cFikrimiz \u015fuydu: V\u00fccuda enjekte edilecek bu proteinler, sitokin f\u0131rt\u0131nas\u0131 taraf\u0131ndan \u00fcretilmi\u015f a\u015f\u0131r\u0131 sitokinlere ba\u011flans\u0131n ve bu a\u015f\u0131r\u0131 stikonleri uzakla\u015ft\u0131rarak enfeksiyondan kaynaklanan semptomlar\u0131 hafifletsin\u201d diye a\u00e7\u0131kl\u0131yor.<\/p>\n
Ara\u015ft\u0131rmac\u0131lar ilk bulgular\u0131n\u0131, Quarterly Review of Biophysics (QRB) Discovery\u2019de (\u00dc\u00e7 Ayl\u0131k Biyofizik Ke\u015fif Yay\u0131n\u0131) yay\u0131mlad\u0131lar ve \u015fimdi geli\u015ftirdikleri proteinleri sitokin sal\u0131m\u0131 ve koronavir\u00fcs enfeksiyonu i\u00e7in hayvan modellerinde ve insan h\u00fccrelerinde test etmeye ba\u015flamay\u0131 umuyorlar.<\/p>\n
\u00c7al\u0131\u015fman\u0131n di\u011fer yazarlar\u0131ndan Shuguang Zhang, MIT Medya Lab\u2019a ba\u011fl\u0131 olarak \u00e7al\u0131\u015fan (Massachusetts Teknoloji Enstit\u00fcs\u00fc\u2019ne ba\u011fl\u0131 disiplinler aras\u0131 bir ara\u015ft\u0131rma laboratuvar\u0131) Molek\u00fcler Mimari Laboratuvar\u0131\u2019ndan bir ara\u015ft\u0131rmac\u0131. Shilei Hao, MIT\u2019te konuk biliminsan\u0131 ve \u00e7al\u0131\u015fman\u0131n ba\u015fyazar\u0131. David Jin ise Avalon GloboCare\u2019in Ba\u015fkan\u0131 ve CEO\u2019su.<\/p>\n
Molek\u00fcler bir s\u00fcnger<\/strong> Problem \u00fczerinde birka\u00e7 y\u0131l \u00e7al\u0131\u015ft\u0131ktan sonra, Zhang ve Qing, bu proteinlerin hidrofobik (sudan korkan) b\u00f6lgelerini de\u011fi\u015ftirmek i\u00e7in bir y\u00f6ntem geli\u015ftirdiler; b\u00f6ylelikle onlar\u0131 suda \u00e7\u00f6z\u00fcnmelerini sa\u011flayacak ve \u00e7al\u0131\u015f\u0131lmas\u0131 daha kolay hale getireceklerdi. QTY kod ad\u0131n\u0131 verdikleri y\u00f6ntemleri, baz\u0131 hidrofobik aminoasitlerin benzer yap\u0131ya sahip hidrofilik (suyu seven) aminoasitlerle yer de\u011fi\u015fmesi esas\u0131na dayan\u0131r. L\u00f6sin glutamine, izol\u00f6sin ve valin treonine, fenilalanin ise tirozine d\u00f6n\u00fc\u015ft\u00fcr\u00fcl\u00fcr.<\/p>\n QTY kodunu geli\u015ftirmelerini takiben, Jin, sitokin resept\u00f6rleri olarak bilenen proteinlerin suda-\u00e7\u00f6z\u00fcnebilir versiyonlar\u0131n\u0131 tasarlama fikri i\u00e7in Zhang\u2019\u0131n laboratuvar\u0131yla g\u00f6r\u00fc\u015ft\u00fc. Bu resept\u00f6rler, iltihab\u0131 ve di\u011fer ba\u011f\u0131\u015f\u0131kl\u0131k tepkilerini uyaran sinyal proteinleri olan sitokinlerin ba\u011f\u0131\u015f\u0131kl\u0131k h\u00fccrelerinin y\u00fczeyinde ba\u011fland\u0131klar\u0131 yerde bulunur.<\/p>\n Jin, sitokin resept\u00f6rlerini taklit eden bu proteinlerin, HIV ve hepatit de dahil olmak \u00fczere, viral ya da bakteriyel enfeksiyonlar taraf\u0131ndan \u00fcretilebilen sitokin f\u0131rt\u0131nalar\u0131yla sava\u015fmaya yard\u0131mc\u0131 olabilece\u011fine inan\u0131yordu. Sitokin f\u0131rt\u0131nalar\u0131 ayn\u0131 zamanda kanser imm\u00fcnoterapisinin bir yan etkisi olarak da ortaya \u00e7\u0131kabilir.<\/p>\n Nisan 2019\u2019da, Zhang\u2019\u0131n ekibi, a\u015f\u0131r\u0131 sitokinleri emebilecek proteinleri tasarlamaya koyuldu. Bunu yapmak i\u00e7in, QTY kodunu kullanarak, sitokin resept\u00f6rlerinin suda \u00e7\u00f6z\u00fcn\u00fcr versiyonlar\u0131n\u0131 \u00fcrettiler. Proteinler suda \u00e7\u00f6z\u00fcn\u00fcr olduklar\u0131nda, insan\u0131n kan dola\u015f\u0131m\u0131nda etkili bir \u015fekilde dola\u015fabilirler; proteinlerin orijinal, hidrofobik versiyonlar\u0131, kar\u015f\u0131la\u015ft\u0131klar\u0131 h\u00fccrelere tutunmak isteyecektir.<\/p>\n Ara\u015ft\u0131rmac\u0131lar, suda \u00e7\u00f6z\u00fcnebilir protein resept\u00f6rlerinin FC b\u00f6lgesi ad\u0131 verilen b\u00f6lgesine bir antikor par\u00e7as\u0131 da eklediler. Bu b\u00f6lge, kan dola\u015f\u0131m\u0131 i\u00e7indeyken proteinlerin daha da stabilize olmas\u0131na yard\u0131m eder ve ba\u011f\u0131\u015f\u0131kl\u0131k sistemi taraf\u0131ndan sald\u0131r\u0131ya u\u011frama olas\u0131l\u0131klar\u0131n\u0131 d\u00fc\u015f\u00fcr\u00fcr.<\/p>\n Ara\u015ft\u0131rmac\u0131lar, kemokin ad\u0131 verilen sitokinle birlikte, interferon ve interl\u00f6kin gibi sitokinlere de ba\u011flanabilen, alt\u0131 ayr\u0131 sitokin resept\u00f6r\u00fcn\u00fc taklit eden proteinleri tasarlad\u0131lar. Laboratuvarda, proteinlerin ba\u011flanma g\u00fcc\u00fcn\u00fc test ettiklerinde, \u00fcrettikleri de\u011fi\u015ftirilmi\u015f proteinlerin, do\u011fal olarak olu\u015fan sitokin resept\u00f6rlerine benzer bir g\u00fc\u00e7le sitokinlere ba\u011flanabildi\u011fini g\u00f6rd\u00fcler.<\/p>\n Jin, \u201cTasarlad\u0131\u011f\u0131m\u0131z sitokin resept\u00f6rleri sitokin f\u0131rt\u0131nas\u0131 s\u0131ras\u0131nda sal\u0131nan a\u015f\u0131r\u0131 sitokinlerin \u00e7o\u011funu emecek\u201d diyor.<\/p>\n Merak\u0131n pe\u015finde<\/strong> Zhang, bu yakla\u015f\u0131m\u0131n potansiyel yararl\u0131l\u0131\u011f\u0131n\u0131n, \u201cmerak\u0131n y\u00f6nlendirdi\u011fi ara\u015ft\u0131rman\u0131n\u201d \u00f6neminin alt\u0131n\u0131 \u00e7izdi\u011finin belirtiyor. \u201cG\u00f6r\u00fcnen o ki, Nisan 2019\u2019da ba\u015flat\u0131lan ara\u015ft\u0131rmam\u0131z, COVID-19 ile enfekte hastalar\u0131n tedavisiyle do\u011frudan alakal\u0131 hale geldi. Merak\u0131n g\u00fcd\u00fcledi\u011fi hatta proaktif (ileriye etkili) ara\u015ft\u0131rmalar da, \u00e7o\u011funlukla, gelecekteki felaketleri \u00f6nlemek i\u00e7in anahtar olabilir, haz\u0131rl\u0131kl\u0131 olmam\u0131za yol a\u00e7abilir.<\/p>\n Ara\u015ft\u0131rmac\u0131lar, suda \u00e7\u00f6z\u00fcn\u00fcr sitokin resept\u00f6rleri olu\u015fturma konusundaki genel yakla\u015f\u0131mlar\u0131 ve tasarlad\u0131klar\u0131 proteinler i\u00e7in patent ba\u015fvurusunda bulundu. Bu teknolojnin lisans\u0131n\u0131 h\u0131zl\u0131 bir \u015fekilde almay\u0131 ve klinik testlere y\u00f6nelmelerine yard\u0131mc\u0131 olabilecek ila\u00e7 ve biyoteknoloji \u015firketleriyle i\u015fbirli\u011fi yapmay\u0131 umuyorlar.<\/p>\n Jin, \u201cA\u00e7\u0131k\u00e7as\u0131 yakla\u015f\u0131m\u0131m\u0131z daha fazla hayvan ve potansiyel insan klinik \u00e7al\u0131\u015fmalar\u0131na gereksinim duyuyor\u201d diyor ve ekliyor, \u201cAma ke\u015ffimizin sitokin f\u0131rt\u0131nalar\u0131 i\u00e7eren viral hastal\u0131klarda kullan\u0131labilecek klinik uygulamalar\u0131 olaca\u011f\u0131na g\u00fcveniyoruz.\u201d Kaynak:<\/strong> http:\/\/news.mit.edu\/2020\/proteins-cytokine-storms-covid-19-0416<\/p>\n","protected":false},"excerpt":{"rendered":" Massachusetts Teknoloji Enstit\u00fcs\u00fc (MIT) Haber B\u00fcrosu\u2019ndan Anne Trafton\u2019un kaleme ald\u0131\u011f\u0131 ara\u015ft\u0131rma haberine g\u00f6re, MIT\u2019ten bir bilim ekibi, koronavir\u00fcs ve di\u011fer enfeksiyonlar\u0131n tedavisinde kullan\u0131labilecek bir strateji olarak, sitokinlere ba\u011flanabilen antikor benzeri resept\u00f6r proteinleri tasarlad\u0131. COVID-19\u2019un belirleyici \u00f6zelliklerinden biri, ciddi vakalarda a\u015f\u0131r\u0131 ba\u011f\u0131\u015f\u0131kl\u0131k yan\u0131t\u0131 ortaya \u00e7\u0131karabilmesidir. Ayn\u0131 zamanda sitokin f\u0131rt\u0131nas\u0131 olarak da bilinen bu a\u015f\u0131r\u0131 ba\u011f\u0131\u015f\u0131kl\u0131k yan\u0131t\u0131 […]<\/p>\n","protected":false},"author":470,"featured_media":42700,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[19],"tags":[257,5848,5026,5763,6292],"class_list":["post-42702","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bilim-gundemi","tag-biyofizik","tag-covid-19","tag-hiv","tag-koronavirus","tag-sitokin-firtinasi"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/posts\/42702","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/users\/470"}],"replies":[{"embeddable":true,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/comments?post=42702"}],"version-history":[{"count":0,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/posts\/42702\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/media\/42700"}],"wp:attachment":[{"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/media?parent=42702"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/categories?post=42702"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/tags?post=42702"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nAra\u015ft\u0131rmac\u0131lar\u0131n sitokin f\u0131rt\u0131nalar\u0131n\u0131 engelleme \u00e7al\u0131\u015fmalar\u0131, Zhang\u2019\u0131n on y\u0131l \u00f6nce ba\u015flad\u0131\u011f\u0131, zara g\u00f6m\u00fcl\u00fc proteinlerin de\u011fi\u015ftirilmi\u015f versiyonlar\u0131n\u0131 geli\u015ftirmeye giri\u015fti\u011fi bir projeden temellendi. Bu proteinleri incelemek genel olarak zordur, \u00e7\u00fcnk\u00fc h\u00fccre zar\u0131ndan \u00e7\u0131kart\u0131ld\u0131ktan sonra, sadece \u00f6zel bir deterjan tipi i\u00e7inde s\u00fcspanse edilirlerse (\u00e7\u00f6z\u00fcnmeden as\u0131l\u0131 kal\u0131rlarsa) yap\u0131lar\u0131n\u0131 korurlar.<\/p>\n
\nGe\u00e7ti\u011fimiz Mart ay\u0131nda, kan\u0131tlar SARS-CoV-2 vir\u00fcs\u00fcn\u00fcn baz\u0131 hastalarda sitokin f\u0131rt\u0131nas\u0131n\u0131 tetikledi\u011fini i\u015faret etmeye ba\u015flad\u0131\u011f\u0131nda, ara\u015ft\u0131rmac\u0131lar tasarlad\u0131klar\u0131 resept\u00f6r proteinlerin bu duruma yard\u0131mc\u0131 olabilece\u011fini fark ettiler. O zamana dek \u00fcrettikleri sonu\u00e7lar\u0131 h\u0131zl\u0131ca yay\u0131mlamaya karar verdiler ve \u015fimdi COVID-19 enfeksiyonlu hayvan modellerinde ve insan h\u00fccrelerinde ek testler yapmay\u0131 planl\u0131yorlar.<\/p>\n
\nAra\u015ft\u0131rma Avalon GloboCare ve \u00c7in Chongqing \u00dcniversitesi ve \u00c7in Burs Kurulu taraf\u0131ndan finanse edilmi\u015f.<\/p>\n