{"id":46509,"date":"2020-09-30T03:34:42","date_gmt":"2020-09-30T00:34:42","guid":{"rendered":"https:\/\/bilimvegelecek.com.tr\/?p=46509"},"modified":"2020-09-30T03:38:37","modified_gmt":"2020-09-30T00:38:37","slug":"yaslanma-ile-iliskili-bir-enzimin-yikim-mekanizmasi-aydinlatildi-yasa-bagli-hastaliklar-ve-bagisiklik-dususu-onlenebilir-mi","status":"publish","type":"post","link":"https:\/\/bilimvegelecek.com.tr\/index.php\/2020\/09\/30\/yaslanma-ile-iliskili-bir-enzimin-yikim-mekanizmasi-aydinlatildi-yasa-bagli-hastaliklar-ve-bagisiklik-dususu-onlenebilir-mi","title":{"rendered":"Ya\u015flanma ile ili\u015fkili bir enzimin bozulma mekanizmas\u0131 ayd\u0131nlat\u0131ld\u0131"},"content":{"rendered":"

Pennsylvania \u00dcniversitesi Perelman T\u0131p Fak\u00fcltesi’nden ara\u015ft\u0131rmac\u0131lar, otofaji (h\u00fccrenin ya\u015flanm\u0131\u015f ve bozulmu\u015f yap\u0131lar\u0131n\u0131n, h\u00fccrenin kendi lizozomu taraf\u0131ndan sindirilmesi) s\u00fcreci ile \u00f6m\u00fcr uzunlu\u011fuyla ili\u015fkili oldu\u011fu bilinen SIRT1 enzimi aras\u0131ndaki ili\u015fkiyi ara\u015ft\u0131rd\u0131.<\/p>\n

Nature Cell Biology<\/em>\u2019de yay\u0131nlanan ara\u015ft\u0131rmada odaklan\u0131lan enzimin bozulma mekanizmas\u0131n\u0131n anla\u015f\u0131lmas\u0131, ya\u015fa ba\u011fl\u0131 hastal\u0131klarla m\u00fccadelede yeni ila\u00e7lar\u0131n ve yeni tedavi metotlar\u0131n kullan\u0131lmas\u0131n\u0131 sa\u011flayabilir. Doktora sonras\u0131 ara\u015ft\u0131rmac\u0131 Lu Wang, \u201cBu enzimatik yolun y\u0131k\u0131m\u0131n\u0131 \u00f6nlemek, ya\u015fa ba\u011fl\u0131 hastal\u0131klar\u0131n ve ba\u011f\u0131\u015f\u0131kl\u0131k d\u00fc\u015f\u00fc\u015f\u00fcn\u00fcn \u00f6nlenmesine, hastal\u0131klar\u0131n tedavi edilmesine yard\u0131mc\u0131 olmak i\u00e7in SIRT1 enziminin d\u00fczeyini eski haline getirmeyi ama\u00e7layan ba\u015fka bir yakla\u015f\u0131m olabilir\u201d dedi ve ekledi: \u201cOtofajinin ba\u011f\u0131\u015f\u0131kl\u0131k h\u00fccrelerinde, SIRT1’deki rol\u00fc daha \u00f6nce g\u00f6sterilmemi\u015ftir. Bu nedenle sonu\u00e7lar belki de en \u00e7ok imm\u00fcn (ba\u011f\u0131\u015f\u0131kl\u0131k) ya\u015flanma alan\u0131 ile ilgili olacakt\u0131r. Otofaji mekanizmas\u0131, ba\u011f\u0131\u015f\u0131kl\u0131k fonksiyonunu eski durumuna getirmek i\u00e7in bize yeni bir olas\u0131l\u0131k sunuyor.\u201d<\/p>\n

\"\"
SIRT1 enziminin yap\u0131s\u0131.<\/figcaption><\/figure>\n

V\u00fccut h\u00fccreleri, ya\u015flanma s\u00fcrecinde SIRT1 \u00f6rne\u011findeki gibi, proteinleri ve enzimleri s\u0131zd\u0131ran musluklar gibi \u00e7al\u0131\u015f\u0131r. Bu nedenle kronik hastal\u0131klar, organlarda fonksiyon kay\u0131plar\u0131 ve enfeksiyonlara kar\u015f\u0131 zay\u0131f ba\u011f\u0131\u015f\u0131kl\u0131k tepkileri ortaya \u00e7\u0131kabilir. Daha \u00f6nceki \u00e7al\u0131\u015fmalar bu s\u0131z\u0131nt\u0131lar\u0131 durdurman\u0131n ve SIRT1 enzimini yenilemenin yeni yollar\u0131 olabilece\u011fini g\u00f6stermi\u015ftir ancak yeni ara\u015ft\u0131rma, ya\u015flanma s\u00fcrecinde otofajinin rol\u00fcn\u00fc g\u00f6steren ilk \u00e7al\u0131\u015fmad\u0131r.<\/p>\n

SIRT1 enzimine odaklanan \u00f6nceki \u00e7al\u0131\u015fmalar, enzimin h\u00fccre metabolizmas\u0131 ve ba\u011f\u0131\u015f\u0131kl\u0131k tepkileri i\u00e7in olduk\u00e7a \u00f6nemli oldu\u011funu, enzimin -enzimin \u00fcretiminde etkili olan genin- anlat\u0131m\u0131 art\u0131r\u0131ld\u0131\u011f\u0131nda \u00f6m\u00fcr uzunlu\u011funda art\u0131\u015f oldu\u011funu g\u00f6stermekteydi. Otofaji ile SIRT1 enzimi aras\u0131ndaki ili\u015fkinin incelendi\u011fi yeni \u00e7al\u0131\u015fmada ise ara\u015ft\u0131rmac\u0131lar, ya\u015flanma s\u00fcreci (senescence) s\u0131ras\u0131nda SIRT1 enziminin aktivite kayb\u0131n\u0131n mekanizmas\u0131n\u0131 belirlemek i\u00e7in, farelerden elde edilen h\u00fccrelerde RNA dizileme tekniklerini kullanarak mRNA sentezi ve kararl\u0131l\u0131\u011f\u0131 (stabilite) ile gen anlat\u0131m\u0131n\u0131n kontrol\u00fcnde etkili olan \u00f6nemli fakt\u00f6rleri denklemden \u00e7\u0131kard\u0131. B\u00f6ylelikle otofaji ile enzim aras\u0131ndaki ili\u015fkiyi do\u011frudan inceleme f\u0131rsat\u0131 elde etti. S\u00fcreci takip eden di\u011fer deneylerde ya\u015flanm\u0131\u015f h\u00fccrelerde otofaji ile ili\u015fkili olan Atg7 proteininin mekanizmadan \u00e7\u0131kar\u0131lmas\u0131yla SIRT1 seviyelerinin yerinde kald\u0131\u011f\u0131 bulundu. B\u00f6ylece enzim kayb\u0131nda proteazomlar\u0131n de\u011fil, otofajik yolun rol oynad\u0131\u011f\u0131 g\u00f6sterildi. (Proteazomlar, h\u00fccrelerin ba\u011flanamam\u0131\u015f proteinleri atmas\u0131n\u0131 ve \u00f6zelle\u015fmi\u015f proteinlerin deri\u015fiminin kontrol edilmesini sa\u011flayan b\u00fcy\u00fck bir sistemin par\u00e7alar\u0131d\u0131r.)<\/p>\n

\"\"
Bir proteazomun yap\u0131s\u0131.<\/figcaption><\/figure>\n

Farelerin \u00e7e\u015fitli ila\u00e7larla tedavi edilmesi de otofaji ile SIRT1 aras\u0131ndaki ili\u015fkiyi destekledi. H\u00fccrelerdeki proteinlerin par\u00e7alanmas\u0131n\u0131 engelleyen bir proteazom inhibit\u00f6r\u00fc, ya\u015flanan h\u00fccrelerde ve dokulardaki SIRT1 proteinini geri kazanmada ba\u015far\u0131s\u0131z oldu. Aksine, otofaji inhibit\u00f6r\u00fc olan Lys05 ile ger\u00e7ekle\u015ftirilen tedavi, enzimin kayb\u0131n\u0131 \u00f6nledi. B\u00f6ylelikle enzimin lizozomlar taraf\u0131ndan par\u00e7aland\u0131\u011f\u0131 anla\u015f\u0131lm\u0131\u015f oldu. (Lizozomlar h\u00fccre i\u00e7indeki sindirimden sorumludur.)<\/p>\n

Ara\u015ft\u0131rmac\u0131lar, ba\u011f\u0131\u015f\u0131kl\u0131\u011f\u0131 sa\u011flayan h\u00fccrelere de odakland\u0131. Ba\u011f\u0131\u015f\u0131kl\u0131k h\u00fccrelerinde SIRT1 ile otofaji aras\u0131ndaki ili\u015fkinin anla\u015f\u0131lmas\u0131 i\u00e7in, insan don\u00f6r CD8 T h\u00fccrelerini (T h\u00fccreleri, ba\u011f\u0131\u015f\u0131kl\u0131\u011f\u0131n sa\u011flanmas\u0131nda g\u00f6revli h\u00fccrelerdendir) d\u00fc\u015f\u00fck dozdaki Lys05 ve bir proteazom inhibit\u00f6r\u00fc ile tedavi etmeyi denedi, yaln\u0131zca Lys05’in SIRT1 enzim seviyesini art\u0131rd\u0131\u011f\u0131n\u0131 buldu. Sonu\u00e7lar, SIRT1 enziminin, insanlarda T h\u00fccresi ya\u015flanmas\u0131 s\u0131ras\u0131nda \u2013k\u0131smen de olsa- otofaji-lizozom yola\u011f\u0131n\u0131n etkinli\u011fi ile par\u00e7aland\u0131\u011f\u0131n\u0131 g\u00f6stermektedir.<\/p>\n

Ara\u015ft\u0131rmac\u0131lar, ke\u015fif ile ilgili kapsaml\u0131 deneylerine devam ederek ya\u015flanmay\u0131 geciktiren bile\u015fiklerin tasar\u0131m\u0131 i\u00e7in yeni yakla\u015f\u0131mlar geli\u015ftirilebileceklerini d\u00fc\u015f\u00fcn\u00fcyor.<\/p>\n

Kaynak:<\/strong> https:\/\/medicalxpress.com\/news\/2020-09-blocking-enzyme-self-destruction-mitigate-age-related.html<\/p>\n","protected":false},"excerpt":{"rendered":"

Pennsylvania \u00dcniversitesi Perelman T\u0131p Fak\u00fcltesi’nden ara\u015ft\u0131rmac\u0131lar, otofaji (h\u00fccrenin ya\u015flanm\u0131\u015f ve bozulmu\u015f yap\u0131lar\u0131n\u0131n, h\u00fccrenin kendi lizozomu taraf\u0131ndan sindirilmesi) s\u00fcreci ile \u00f6m\u00fcr uzunlu\u011fuyla ili\u015fkili oldu\u011fu bilinen SIRT1 enzimi aras\u0131ndaki ili\u015fkiyi ara\u015ft\u0131rd\u0131. Nature Cell Biology\u2019de yay\u0131nlanan ara\u015ft\u0131rmada odaklan\u0131lan enzimin bozulma mekanizmas\u0131n\u0131n anla\u015f\u0131lmas\u0131, ya\u015fa ba\u011fl\u0131 hastal\u0131klarla m\u00fccadelede yeni ila\u00e7lar\u0131n ve yeni tedavi metotlar\u0131n kullan\u0131lmas\u0131n\u0131 sa\u011flayabilir. Doktora sonras\u0131 ara\u015ft\u0131rmac\u0131 Lu […]<\/p>\n","protected":false},"author":1390,"featured_media":46513,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[19],"tags":[1194,5345,6999,6998,423],"class_list":["post-46509","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bilim-gundemi","tag-bagisiklik","tag-enzim","tag-lizozom","tag-proteazom","tag-yaslanma"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/posts\/46509","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/users\/1390"}],"replies":[{"embeddable":true,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/comments?post=46509"}],"version-history":[{"count":0,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/posts\/46509\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/media\/46513"}],"wp:attachment":[{"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/media?parent=46509"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/categories?post=46509"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bilimvegelecek.com.tr\/index.php\/wp-json\/wp\/v2\/tags?post=46509"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}